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primescripttm reverse transcriptase kit  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm reverse transcriptase kit
    Primescripttm Reverse Transcriptase Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 736 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+ii+reverse+transcriptase+kit/PrimeScript+II+Reverse+Transcriptase/pm41803983-65-14-19
    Average 96 stars, based on 736 article reviews
    primescripttm reverse transcriptase kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Remission of copper-induced liver injury through the PXR/NF-kB signaling pathway: The effects of dietary curcumin supplementation in largemouth bass (Micropterus salmoides).
    Article Snippet: The quality and quantity of RNA were assessed using 1.0 % agarose gel electrophoresis, and the concentration was determined using an Implen NanoPhotometer (Implen Inc., Germany). .. The cDNA was reverse transcribed from 1000 ng of DNAase-free RNA using a PrimeScriptTM II Reverse Transcriptase Kit (Takara, Japan). qRT-PCR assays were performed in a 20 μL reaction system containing 2 μL of diluted cDNA, 0.8 μL of forward and reverse primers (10 μM), 6.4 μL of DEPC water, and 10 μL of 2 × SYBR Premix Ex Taq II (Takara, Japan). ..

    Article Title: Full genome characterization and evolutionary analysis of Banna virus isolated from Culicoides , mosquitoes and ticks in Yunnan, China.
    Article Snippet: Viral genomic segments were separated by 1% agarose gel electrophoresis (AGE) at 90 V for 4 h in 1× Tris-Acetate EDTA (TAE) buffer, stained with Goldview II (Solarbio, Beijing, China), and photographed by a Gel DocTM XR+ System with Image LabTM software (Bio-Rad, Hercules, CA, USA). frontiersin.org 2.4 Full-length amplification of cDNAs and sequencing BAV genomic segments were reverse-transcribed into cDNA using full-length amplification of cDNAs (FLAC) technique as described by Maan et al. (Maan et al., 2007). .. Briefly, the 3’ ends of each viral dsRNA were ligated to an ‘anchor-primer’, with a phosphorylated 5’ terminus, using T4 RNA ligase, followed by reverse transcription using a PrimeScriptTM II Reverse Transcriptase Kit (TaKaRa, Dalian, China). .. The resulting cDNAs were amplified using a primer (5-15-1) complementary to the ‘anchor-primer’ by high-fidelity PrimeSTAR® GXL DNA Polymerase (TaKaRa), the amplicons were analyzed by 1% AGE.

    Article Title: Diversity of classic and novel human astrovirus in outpatient children with acute gastroenteritis in Shanghai, China
    Article Snippet: .. Reverse transcription (RT) was performed using random primers with the PrimeScriptTM II Reverse Transcriptase Kit [Takara, Biotechnology (Dalian) Co., Ltd., China]. ..

    Article Title: CBFA2T2: a novel H3K27 reader regulating metabolism and tumor growth.
    Article Snippet: .. Complementary DNAs were synthesized by PrimeScriptTM II Reverse Transcriptase Kit with gDNA Eraser (TaKaRa, RR047A). .. Libraries with different indexes were multiplexed ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS and loaded onto an Illumina NovaSeq 6000 instrument for sequencing using a 2×150 paired-end (PE) configuration according to manufacturer’s instructions.

    Article Title: CBFA2T2: a novel H3K27 reader regulating metabolism and tumor growth
    Article Snippet: .. Complementary DNAs were synthesized by PrimeScriptTM II Reverse Transcriptase Kit with gDNA Eraser (TaKaRa, RR047A). .. The PCR mixture, prepared in triplicate, consisted of the synthesized cDNA, gene-specific primers, FastStart Universal SYBR Green Master (Rox) (Roche, 4913914001), and was subsequently subjected to LightCycler 480 II Real-Time PCR System (Roche).

    Article Title: Isolation and Genetic Characterization of Three Palyam Serogroup Orbiviruses From Culicoides Spp. and Associated Infections in Cattle in Yunnan, China
    Article Snippet: In brief, the 3′ ends of viral dsRNA were ligated to an anchor primer and purified using a Monarch PCR & DNA Cleanup kit (NEB, USA). .. Then, cDNA was synthesized using the PrimeScriptTM II Reverse Transcriptase kit (Takara, Dalian, China). .. The resulting cDNAs were amplified using the primer 5-15-1 [ ] by PrimeSTAR GXL DNA Polymerase (TaKaRa, Dalian, China).

    Article Title: Furazolidone reduces the pathogenesis of Trueperella pyogenes and Pseudomonas aeruginosa co-infection in a mouse model.
    Article Snippet: Total RNAs from lung of infected mice at indicated time points (day1, day3, day 7, and day 14) were obtained using TRIzol reagents (ThermoFisher). .. The cDNA was synthesized by a PrimeScriptTM II Reverse Transcriptase kit (Takara Bio Inc., Kusatsu, Japan). .. Realtime PCR reactions were performed using TB Green Premix Ex TaqII (Tli RNaseH Plus) Kit (Takara) to detect the relative expression of QS-related genes of PAO1, including lasR、rhlR、pqsR、lasB、rhlA、pqsA、pqsD、pqsE and hcnA.

    Article Title: Full genome characterization and evolutionary analysis of Banna virus isolated from Culicoides , mosquitoes and ticks in Yunnan, China
    Article Snippet: .. Briefly, the 3’ ends of each viral dsRNA were ligated to an ‘anchor-primer’, with a phosphorylated 5’ terminus, using T4 RNA ligase, followed by reverse transcription using a PrimeScriptTM II Reverse Transcriptase Kit (TaKaRa, Dalian, China). .. The resulting cDNAs were amplified using a primer (5-15-1) complementary to the ‘anchor-primer’ by high-fidelity PrimeSTAR® GXL DNA Polymerase (TaKaRa), the amplicons were analyzed by 1% AGE.

    Quantitative RT-PCR:

    Article Title: Remission of copper-induced liver injury through the PXR/NF-kB signaling pathway: The effects of dietary curcumin supplementation in largemouth bass (Micropterus salmoides).
    Article Snippet: The quality and quantity of RNA were assessed using 1.0 % agarose gel electrophoresis, and the concentration was determined using an Implen NanoPhotometer (Implen Inc., Germany). .. The cDNA was reverse transcribed from 1000 ng of DNAase-free RNA using a PrimeScriptTM II Reverse Transcriptase Kit (Takara, Japan). qRT-PCR assays were performed in a 20 μL reaction system containing 2 μL of diluted cDNA, 0.8 μL of forward and reverse primers (10 μM), 6.4 μL of DEPC water, and 10 μL of 2 × SYBR Premix Ex Taq II (Takara, Japan). ..

    Synthesized:

    Article Title: CBFA2T2: a novel H3K27 reader regulating metabolism and tumor growth.
    Article Snippet: .. Complementary DNAs were synthesized by PrimeScriptTM II Reverse Transcriptase Kit with gDNA Eraser (TaKaRa, RR047A). .. Libraries with different indexes were multiplexed ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS and loaded onto an Illumina NovaSeq 6000 instrument for sequencing using a 2×150 paired-end (PE) configuration according to manufacturer’s instructions.

    Article Title: CBFA2T2: a novel H3K27 reader regulating metabolism and tumor growth
    Article Snippet: .. Complementary DNAs were synthesized by PrimeScriptTM II Reverse Transcriptase Kit with gDNA Eraser (TaKaRa, RR047A). .. The PCR mixture, prepared in triplicate, consisted of the synthesized cDNA, gene-specific primers, FastStart Universal SYBR Green Master (Rox) (Roche, 4913914001), and was subsequently subjected to LightCycler 480 II Real-Time PCR System (Roche).

    Article Title: Isolation and Genetic Characterization of Three Palyam Serogroup Orbiviruses From Culicoides Spp. and Associated Infections in Cattle in Yunnan, China
    Article Snippet: In brief, the 3′ ends of viral dsRNA were ligated to an anchor primer and purified using a Monarch PCR & DNA Cleanup kit (NEB, USA). .. Then, cDNA was synthesized using the PrimeScriptTM II Reverse Transcriptase kit (Takara, Dalian, China). .. The resulting cDNAs were amplified using the primer 5-15-1 [ ] by PrimeSTAR GXL DNA Polymerase (TaKaRa, Dalian, China).

    Article Title: Furazolidone reduces the pathogenesis of Trueperella pyogenes and Pseudomonas aeruginosa co-infection in a mouse model.
    Article Snippet: Total RNAs from lung of infected mice at indicated time points (day1, day3, day 7, and day 14) were obtained using TRIzol reagents (ThermoFisher). .. The cDNA was synthesized by a PrimeScriptTM II Reverse Transcriptase kit (Takara Bio Inc., Kusatsu, Japan). .. Realtime PCR reactions were performed using TB Green Premix Ex TaqII (Tli RNaseH Plus) Kit (Takara) to detect the relative expression of QS-related genes of PAO1, including lasR、rhlR、pqsR、lasB、rhlA、pqsA、pqsD、pqsE and hcnA.



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